MSc Thesis , Ph. D , and other potential research


DBT PROJECT BARCODES
Cassia fistula  SDP850029-20 priyanka
Strychnos nux-vomica 19-22947 SDP850021-20 priyanka , Photography: Mandeep and Richa
 Bougainvillea spectabilis     SDP850020-20 PRIYANKA 

Sapindus mukorossi 15-22947 SDP850019-20 priyanka , Photography: Mandeep and Richa

(SDP850093-23 93-101 are from Anita botany)

 
TITLES OF MSC THESIS DEPOSITED SO FAR

TOPIC

SUBMITTED BY

YEAR

1. Dna isolation from Rosa American pride and amplication of rbcl gene

Neeraj kumar goyal

2016

2. Dna barcoding of Rosa

burnaby

Ashima Chadha

2016

3. Dna isolation of Rosa Ice Berg and amplification of RBcl gene

Vishakha Sharma

2016

4. Dna isolation from Rosa minnie pearl amplification

of rbcl gene using PCR

Asheesh Maihla

2016

5. Dna isolation from the Plant Rosa bankside and Amplication of matK/RBCL Gene by using PCR

Kanchan Dogra

2017

6. Dna isolation from Rosa Ingrid Bergmann & amp-

Lification of RBcl & matK gene

Kavita Dhiman


2017

7. Forensic importance of Plant dna barcoding in Crime investigation by Using RBcl gene & matK Gene of Rosa Indica Tajmahal, Rosa Indica Grandmughal (orange), Rosa indica grandmughal (hybrid) & nerium oleander

Geetanjali Vij

2017

8. Dna isolation from Rosa Grandiflora, PCR amplification and dna sequencing of RBcl & matK gene

Navpreet Kaur

2017

9. Dna isolation from Rosa Sentimental, amplification of RBcl gene & matK gene and Dna sequencing

Rishibha Jain

2017

10. Dna isolation from Rosa Webbiana and amplification

Of of RBcl gene

Mayank

2017

11. Dna isolation, amplification of RBcl gene from Catharanthus roseus using PCR and sequencing for dna

Barcoding

Trupti Rajkumar

Kanekar

2017 low sequence quality

12. Dna isolation from Rosa Christian dior and amplification of rbcl gene using PCR technique

Nitika

2018

13. Dna isolation from Rosa Papa meilland and ampli-

fication of RBcl gene using PCR

Harmandeep kaur

2018

14 Plant Dna barcoding in Crime investigation by Using RBcl gene for Rosa Floribunda Var Zorina

Poonam

2018

15. Dna isolation from Rosa Queen Elizabeth and amplification of RBcl gene using PCR

Lalhruaitluangi

2018

16. Dna isolation from floribunda var angel face, PCR amplification and dna Sequencing of RBcl gene

Lakshay Kalra

2018

17. Dna isolation from Calotropis Procera and amplification of RBcl gene using PCR technique

Anjali Sood

2019 done

18. Dna isolation, amplification of RBcl gene from Poppy seeds (papaver somniferum) using PCR and Sequencing for Dna barcoding

Aradhna Karma

2019 done SDP850004-19

19. DNA BARCODING OF Abrus Precatorius and Vitex

Nigundo PLANT: APPLICATION TO FORENSIC ANALYSIS

Sukhpreet kaur

2019 done SDP850005-19

20. DNA ISOLATION FROM ALOE BARBADENSIS MILLER AND AMPLIFICATION OF RBCL GENE (DNA BARCODING REGION) AND ITS APPLICATION TO FORENSIC ANALYSIS

NIDHI ELIZABETH HANSDAK

2019 done (SDP850022-20)

21. Calotropis Gigantica

Neelanchal

2019

22. DNA Barcoding of three plant Rosa species- Rosa painter, Rosa pride of Cheshire and Rosa milky way

VAIBHAV PRAKASH

2020 online

23. DNA ISOLATION FROM ROSA FLORIBUNDA VAR.ETERNAL FLAME ROSE, PCR APMLIFIVCATION AND GEL ELECTROPHORESIS

SMAILIE ATRI

2020 online

24. DNA isolation and Gel Electrophoresis of plant samples of Rosa species- Rosa ‘Quo Vadis’ and Rosa ‘Sun Goddess’

BABITA

2020 online

25. ISOLATION OF DNA AND AMPLIFICATION OF rbcL GENE USING PCR TECHNIQUE FROM PLANT ROSA BLACK RUBY AND ROSA GEMINI

SHIVANI

2020 online

26. DNA EXTRACTION,AMPLIFICATION OF RBCL GENE FROM TWO PLANT SAMPLES OF ROSA SPECIES–Rosa president giri and Rosa twice in blue USING PCR AND GEL ELECTROPHORESIS FOR DNA BARCODING

KUNZES DOLMA

2020 online

27. ISOLATION OF DNA FROM ROSA NIGHT TIME AND ROYAL DOT PLANT AND AMPLIFICATION OF rbcl GENE BY PCR TECHNIQUE AND GEL ELECTROPHORESIS FOR DNA BARCODING

DIVYA KATOCH

2020 online

28. DNA ISOLATION, AMPLIFICATION OF RBCL GENE FROM ROSA LAGERFELD AND MADAM DELBARD PLANT USING PCR AND GEL ELECRTOPHORESIS FOR DNA BARCODING

NAKSHATRA SINGH

2020 online

29. DNA BARCODING OF CYCAS REVOLUTA ( SAGO PALM) IN CRIME SCENE INVESTIGATION

AKANKSHA TYAGI

2021 low sequence quality

30. DNA BARCODING OF DOLICHANDRA UNGUIS-CATI FOR FORENSIC INVESTIGATION

VANDANA RANI

2021

31. DNA Barcoding of Casuarina equisetifolia

HARPREET KAUR KAHLON

2021

32. DNA Barcoding of plant Zamia furfuracea

LORIC

2021

33. DNA Barcoding of Ginkgo biloba in criminal investigation

IKSHIKA SHARELY

2021 low sequence quality

34. Plant DNA Barcoding of Datura innoxia

NANDINI SHARMA


2021 low sequence quality

35. PLANT DNA BARCODING OF TAXODIUM HUEGILII (TAXODIUM MUCRONATUM) IN CRIME INVESTIGATION

PRIYA RANI

2021 pcr low amount

36. DNA BARCODING OF THE MEDICINAL PLANT,

CRATEVA RELIGIOSA (SACRED BARNA)

ANMOLDEEP SINGH SANDHU

2021 low sequence quality

37. FORENSIC IMPORTANCE OF PLANT DNA BARCODING OF LITCHI CHINENSIS PLANT BY USING MATK GENE

RICHA SHARMA

2022

low sequence quality

38. DNA BARCODING OF PLANT Justicia brandegeeana

ABHISHEK THAKUR

2022 submitted in bold but need to redo

39. PLANT DNA BARCODING OF Brunfelsia pauciflora - AN AID IN FORENSIC ANALYSIS

VIBHUTI

2022 submitted in bold but need to redo

40. PLANT DNA BARCODING OF PLANT- Euphorbia milli

MASOOM KAUSHAL


2022 low sequence quality

41. PLANT DNA BARCODING BY USING MATK GENE FOR - Pterygota alata

MANDEEP KAUR

2022 low sequence quality

42. Plant DNA Barcoding by using matK gene for Aglaonema modestum

BHAVJEETSINGH BAJWA

2022 pcr amount low

43. DNA BARCODING OF MORUS ALBA (SHAHTOOT)

KHUSHBOO

2022 submitted in bold but need to redo

44. DNA BARCODING OF WISTERIA SINENSIS

45 PLANT DNA BARCODING OF Juniperus formosana and Nymphaea mexicana 

 

46 PLANT DNA EXTRACTION AND BARCODING: FROM EXTRACTION METHODS TO FORENSIC ANALYSIS

 

47 DNA BARCODING OF PLANTS - Ixora chinensis, Melaleuca pachyphylla and Pyrostegia venusta

 

48 DNA BARCODING OF Ficus krishnae, Schleichera trijuga &Pereskia grandiflora

 

49  DNA barcoding of various plant species using

30 seconds DNA extraction method

50 PLANT DNA BARCODING OF Tabernaemontana orientalis and Millingtonia hortensis

51 PLANT DNA BARCODING of Plant Ipomoea carnea and Euphorbia grantii








52 Plant DNA barcoding of Diaspyros embryopteris and Sansevieria zeylanica.


53. Plant DNA barcoding of Lysimachia foemina and  Sansevieria trifasciata

54. Plant DNA barcoding of Sansevieria cylindrica and Justicia brandegeeana 

55. Plant DNA barcoding of sansevieria cylindrica and Matthiola incana

56. Plant DNA barcoding of Cleratum bellidiforme , Ficus krishnae and Sansevieria zeylanica  

57. Plant DNA barcoding of Ravenia spectabilis and Sansevieria trifasciata

58. Plant DNA barcoding of Cassia siamea and Sansevieria kirki (due) 

 

Riya Garg

 NEERAJ KUMAR SINGLA

 

 

SHARU RANA 

 


MANPREET KAUR

 

 

STANZIN YANGCHAN

 


NANCY SHARMA


ANSHUL

 

DIKSHA THAKUR







Priya Thakur


Prashant Mehra


Jugraj Singh

Ayushi Sharma


Ghanshyam

Akshita Jokta

Nitika Singh  (due)

2022 submitted in bold but need to redo

2023 www.boldsystems.org/index.php/Public_RecordView?processid=SDP850084-23

www.boldsystems.org/index.php/Public_RecordView?processid=SDP850083-23

2023 WORKED ON Ixora acuminata
and Cassia siamea.

www.boldsystems.org/index.php/Public_RecordView?processid=SDP850070-23

2023 www.boldsystems.org/index.php/Public_RecordView?processid=SDP850068-23

www.boldsystems.org/index.php/Public_RecordView?processid=SDP850072-23

2023 www.boldsystems.org/index.php/Public_RecordView?processid=SDP850067-23

www.boldsystems.org/index.php/Public_RecordView?processid=SDP850079-23

www.boldsystems.org/index.php/Public_RecordView?processid=SDP850073-23

 

2023

2023

2023 www.boldsystems.org/index.php/Public_RecordView?processid=SDP850074-23

 Sansevieria zeylanica trnl

2024

Sansevieria trifasciata
rbcl 
2024

Sansevieria cylindrica
Rbcl 
2024

Sansevieria cylindrica
trnl  
2024


Sansevieria zeylanica  
rbcl  
2024
Sansevieria trifasciata
trnl 
2024
Sansevieria kirki
trnl   
2024 (due)

 Literature suggested for reading for research on DNA barcoding:
Essential:
http://www.dnabarcoding101.org/
http://www.dnabarcoding101.org/files/using-dna-barcodes.pdf
http://www.dnabarcoding101.org/resources/index.html

http://www.ibol.org/phase1/about-us/what-is-dna-barcoding/
http://www.ibol.org/phase1/about-us/what-is-ibol/http://www.ibol.org/phase1/about-us/campaigns/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909793/
https://www.boldsystems.org/index.php/Resources/whatIsBOLD
http://www.barcodeoflife.org/content/partners?perpage=20
http://www.barcodeoflife.org/sites/default/files/materials/BOLI_Brochure_Final.pdf
http://www.sciencedirect.com/science/article/pii/S1875176815301037
https://web.archive.org/web/20180322200442/http://chandigarhforestflora.in/

Advanced:
TOPIC NAME AUTHOR NAME YEAR WEBSITE LINK & PubMed ID
1. Application of plant DNA markers in forensic botany: Genetic comparison of Quercus evidence leaves to crime
scene trees using microsatellites
Kathleen J. Craft , Jeffrey D. Owens , Mary V. Ashley 2 MAY 2006 https://www.sciencedirect.com/science/article/pii/S0379073806001356 & PMID: 16632287
2. Identification of ethnomedicinal plants (Rauvolfioideae: Apocynaceae) through
DNA barcoding from northeast India
PradoshMahadani, GouriDuttaSharma,and Sankar Kumar Ghosh 11 JUNE 2013 http://www.phcog.com/text.asp?2013/9/35/255/113284 & PMID: 23930011
3. DNA BARCODING RESEARCH IN INDIA : EXCITEMENT AND DEVELOPMENT
Wazir S. Lakra, M. Singh, M. Goswami,
A. Gopalkrishnan and S. Ayyappan
13 MAY 2010 http://www.dnabarcodes2011.org/documents/presentations/12-2/taxsessionsa/fish1/915-Lakra.pdf
4. DNA barcoding: The hi-tech fight against fake food Rose Eveleth 10 SEPT 2012 http://www.bbc.com/future/story/20120907-hi-tech-fight-against-fake-food
5. A modified method to isolate genomic DNA from plants without liquid
nitrogen
KAKOLI BISWAS &
RAJESH BISWAS
15 APRIL 2011 https://www.researchgate.net/profile/Kakoli_Biswas/publication/235663237_A_modified_method_to_isolate_DNA_from_Plants_without_Liquid_Nitrogen_Current_Science_Vol_10011_1622-1624/links/00b7d5339abba8e3d7000000.pdf?origin=publication_detail
6. Science Needs for Microbial Forensics: Initial International
Research Priorities
National Academy of Sciences 2014 http://www.nap.edu/catalog.php?record_id=18737 & PMID: 25144023
7. CCDB Primer sets plants Maria Kuzmina and Natalia 2016 http://ccdb.ca/site/wp-content/uploads/2016/09/CCDB_PrimerSets-Plants.pdf
8. DNA Barcoding Methods for Land Plants
Aron J. Fazekas , Maria L. Kuzmina , Steven G. Newmaster ,
and Peter M. Hollingsworth
2012
https://www.researchgate.net/publication/225287511_DNA_Barcoding_Methods_for_Land_Plants &
PMID:  22684959
9.ChargeSwitch® gDNA Plant Kit
For purification of genomic DNA (gDNA) from
plant samples
User manual 13 NOV 2006 http://tools.thermofisher.com/content/sfs/manuals/chargeswitch_plant_man.pdf
10. Chloroplast genome sequences from total DNA for plant
identification
Catherine J. Nock1, Daniel L.E. Waters1, Mark A. Edwards1, Stirling G. Bowen1, Nicole Rice1,2, Giovanni
M. Cordeiro1 and Robert J Henry
8 JUNE 2010 http://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2010.00558.x/full & PMID: 20796245
11. A Comparison of DNA Extraction Methods using Petunia hybrida Tissues FarshadTamari,Craig S. Hinkley, and NaderiaRamprashad 3 SEPT 2013
https://www.researchgate.net/profile/Craig_Hinkley/publication/256334191_A_Comparison_of_DNA_Extraction_Methods_using_Petunia_hybrida_Tissues/links/559c7bae08ae0035df248220.pdf
PMID: 23997658
12. DNA Barcoding in plants:taxonomy in a new perspective K.Vijayan and C.H.Tsou 10 DEC 2010

13.Direct PCR amplification from leaf discs H.J. Rogers ,H.C. Parkes 22 MAR 1999 https://www.sciencedirect.com/science/article/pii/S0168945299000485
14.Molecular Diversity and DNA Barcode Identification of Selected Philippine Endemic Hoya Species (Apocynaceae) Faith S. Maranan,and Ma. Genaleen Q. Diaz MAR 2013 https://www.researchgate.net/publication/282713626_Molecular_Diversity_and_DNA_Barcode_Identification_of_Selected_Philippine_Endemic_Hoya_Species_Apocynaceae
15.
DNA extraction: Comparison of methodologies

Ambika B Gaikwad


https://www.scribd.com/document/290953477/DNA-Extraction-Comparison-of-Methodologies
16.Forensic Botany: An Emerging Discipline of Plant Sciences Rabish Chandra and Vinny Sharma 2014 http://www.indianbotanists.com/2014/03/forensic-botany-emerging-discipline-of.html
17.DNA Barcode Standards
New community standards adopted for fungi and protists
International barcode of life 3 DEC 2012 http://ibol.org/wp-content/uploads/2012/12/iBOL-Barcode-Bulletin-Dec-2012.pdf
18.Using FTA card in DNA samples Whatman ltd. --- http://parliament.vic.gov.au/images/stories/committees/lawrefrom/forensics/submissions/Forensics-Whatman_2.pdf
19.GenElute Plant Genomic
DNA Miniprep Kit
User guide --- http://emidioalbertini.com/pdf/PLN70.pdf
20.HiPurA™ Plant DNA Isolation Kit (CTAB Method) HiMedia --- https://www.scribd.com/doc/207402236/Cataloguemmm
21.India announces $10M barcoding initiative
Focus on plants, insects and fishes
Director General of ICAR 17 FEB 2016 http://ibol.org/india-announces-10m-barcoding-initiative/
22.Identifying personal microbiomes using metagenomic codes Eric A. Franzosa,KatherineHuang,James F. Meadow& Many more authors 6 APR 2015 http://www.pnas.org/content/112/22/E2930.full.pdf & PMID :25964341
23.Leaf-punch method to prepare a large number of PCR
templates from plants for SNP analysis
Sachiko Shiokai ,Hiroyasu Kitashiba,
Kenta Shirasawa,Kuniaki Nagano &
Takeshi Nishio
7 DEC 2008 https://link.springer.com/content/pdf/10.1007%2Fs11032-008-9244-9.pdf
24. Leaf disc Template:Alkali Treatment of Plant Tissues for PCR Template ------- ---- http://www.cmglab.usask.ca/Resources%20and%20Protocols%20/Leaf%20Disk%20Template/index.pdf
25.New universal matKprimers for DNA barcoding angiosperms Jing YU ,Jian-Hua XUE ,Shi-Liang ZHOU 8 MAR 2011 http://onlinelibrary.wiley.com/doi/10.1111/j.1759-6831.2011.00134.x/full
26.Universal MultiplexablematKPrimers for DNA Barcoding of Angiosperms Jacqueline Heckenhauer, Michael H. J. Barfuss, and Rosabelle Samuel

2016
http://www.bioone.org/doi/10.3732/apps.1500137 & PMID:27347449
27.DNA barcoding: a practical guide Martyn Powell, Michelle van der Bank, Olivier Maurin& Vincent Savolainen ----- https://www.scribd.com/document/160443152/Molecular-web
28.A rapid and cheap protocol for preparation of PCR templates in peanut Chuan Tang Wang,Xiu Zhen Wang 23 JAN 2009 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/viewFile/v12n2-11/706
29.Visualization of Plastids in Pollen Grains: Involvement of
FtsZ1 in Pollen Plastid Division
Lay Yin Tang , Noriko Nagata , Ryo Matsushima, Yuling Chen , Yasushi Yoshioka andWataru Sakamoto 2009 & PMID :19282372
30.Alkali treatment for rapid preparation of plant material for
reliable PCR analysis
Victor Klimyuk, Bernard J. Carroll, Colwyn M.
Thomas and Jonathan D.G. Jones
5 JAN 1993 http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.1993.tb00169.x/abstract & PMID: 82204556
31.DNA BARCODING AS A MEANS FOR IDENTIFYING
MEDICINAL PLANTS OF PAKISTAN
M. SCHORI* AND A.M. SHOWALTER 14 OCT 2011 http://www.ohio.edu/plantbio/staff/showalte/showalter-lab/Schori%20and%20Showalter%202011%20Pak%20J%20Bot.pdf
32.CTAB Protocol for Isolating DNA from Plant Tissues ------- --- https://opsdiagnostics.com/notes/protocols/ctab_protocol_for_plants.htm
33.DNA from pollen: principles and potential K.D. Bennett* and L. Parducci 16 MAY 2006 http://www.academia.edu/1279261/DNA_from_pollen_principles_and_potential
34.Genetic study of phenylthiocarbamide (PTC)
taste perception among six human populations
of Jammu and Kashmir (India)
MohdFareed, Ahsana Shah, RuqaiyaHussain, Mohammad Afzal 4 MAR 2012 https://www.sciencedirect.com/science/article/pii/S1110863012000043
35.Discriminating plants using the DNA barcode rbcLb: an
appraisal based on a large data set
WENPAN DONG,TAOCHENG,CHANGHAO LI,CHAO XU, PING LONG,CHUMMING
CHEN and SHILIANG ZHOU
1 OCT 2013 & PMID:24119263
36.Arabidopsis gDNA isolation GNU FDL (OPEN WETWARE) 23 FEB 2009 https://openwetware.org/wiki/Arabidopsis_gDNA_isolation
37.A simple and efficient genomic DNA extraction protocol for large scale
genetic analyses of plant biological systems
KamirouChabiSika,TimnitKefela, Hubert Adoukonou-Sagbadja, Leonard Ahotone, AliouSaidou ,
Lamine Baba-Moussa ,LylaJno Baptiste , Simeon O. Kotconi,EmmaW. Gachomoa
7 MAR 2015 https://www.sciencedirect.com/science/article/pii/S2352407315000086
38. Direct PCR amplification and sequencing of specimens’ DNA from preservative ethanoL

ShadiShokralla, Gregory A. C. Singer, and MehrdadHajibabaei
Biodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada
11 JAN 2010
https://www.biotechniques.com/BiotechniquesJournal/2010/March/Direct-PCR-amplification-and-sequencing-of-specimens-DNA-from-preservative-ethanol/biotechniques-187463.html
& PMID: 20359306
39.TBE buffer ------- 2015 https://en.wikipedia.org/wiki/TBE_buffer
40.The changing epitome of species identification –
DNA barcoding
M. Ajmal Ali ,*, Ga´ borGyulai, Norbert Hidve´ gib, Bala´ zsKerti ,
Fahad M.A. Al Hemaid , Arun K. Pandey , Joongku Lee
23 MAR 2014 https://www.sciencedirect.com/science/article/pii/S1319562X14000321 & PMID:24955007
41.Tissue-direct PCR, a rapid and extraction-free method
for barcoding of ferns
F-W. LI,L-Y. KUO,Y-M. HUANG,W-L. CHIOU and C-N. WANG* 1 JUNE 2009 & PMID: 21564993
42. Universal Plant DNA Barcode Loci May Not Work in
Complex Groups: A Case Study with Indian Berberis
Species
Sribash Roy1, Antariksh Tyagi1, Virendra Shukla1, Anil Kumar, Uma M. Singh1, LalBabuChaudhary,
BhaskarDatt, Sumit K. Bag, Pradhyumna K. Singh, Narayanan K. Nair, Tariq Husain, RakeshTuli
27 OCT 2010 https://www.researchgate.net/profile/Uma_Singh2/publication/47718622_Universal_Plant_DNA_Barcode_Loci_May_Not_Work_in_Complex_Groups_A_Case_Study_with_Indian_Berberis_Species/links/0912f505945889a54a000000.pdf?inViewer=0&pdfJsDownload=0&origin=publication_detail
43.Using DNA Barcodes
to Identify and Classify
Living Things                    
Cold Spring Harbor Laboratory DNA Learning Center 2014 http://www.dnabarcoding101.org/files/using-dna-barcodes.pdf