MFS-T2-C1: Molecular Biology and Biochemistry

UNIT-I

1 Chemistry of Nucleotides, Structure of DNA/RNA


https://en.wikibooks.org/wiki/Structural_Biochemistry/Organic_Chemistry/Nucleic_Acids_(DNA)

Original Watson Crick Paper
 https://www.nature.com/scitable/content/16331/molecularstructureofDNAswatsoncrick.pdf#toolbar=0&zoom=100

optional extra:
 commented Watson Crick Paper http://www.exploratorium.edu/origins/coldspring/printit.html
http://www.nature.com/scitable/topicpage/dna-replication-and-checkpoint-control-in-s-14202419

https://www.nature.com/scitable/topicpage/discovery-of-dna-structure-and-function-watson-397

http://www.rsc.org/Education/Teachers/Resources/cfb/nucleicacids.htm
2 DNA Replication: Eukaryotic and Prokaryotic Helicases, Topoisomerases and other DNA replicating Enzymes


http://www.nature.com/scitable/topicpage/major-molecular-events-of-dna-replication-413

https://www.dnalc.org/resources/3d/04-mechanism-of-replication-advanced.html


https://www.youtube.com/watch?v=I9ArIJWYZHI




3 DNA Denaturation, Cot-Rot curves,
http://faculty.tru.ca/dnelson/courses/biol335/335notes/3recdna/4-hybrization/recDNA3b.html


4 DNA Repair mechanisms
http://www.nature.com/scitable/topicpage/dna-damage-repair-mechanisms-for-maintaining-dna-344 
 
 http://www.youtube.com/watch?v=xS6bJMcOrAk

DNA repair - NHEJ - Canonical Non-homologus end joining pathway 2.0 - Full


http://www.youtube.com/watch?v=72oT6N90iWc

DNA repair : Base excision repair SHORT patch v

http://www.youtube.com/watch?v=9PEdqBuDMHM

Homologous Recombination of Double Strand Breaks

http://www.youtube.com/watch?v=g4khROaOO6c


DNA repair: Base excision repair LONG patch 1.0 -



http://www.youtube.com/watch?v=4CIWfTZVlfg

AP Biology - DNA Ligase and its Function


5 DNA Epigenetics
http://www.nature.com/scitable/topicpage/epigenetic-influences-and-disease-895
 

6 Eukaryotic and Prokaryotic Transcription.

http://www.nature.com/scitable/topicpage/dna-transcription-426RNA Processing and Editing


http://www.youtube.com/watch?v=WsofH466lqk


Transcription



http://www.youtube.com/watch?v=7ZpZ4fu7PAU


GENETICS2: TRANSCRIPTION TERMINATION

 http://www.youtube.com/watch?v=SMtWvDbfHLo

DNA Transcription (Advanced)

7 RNA Processing and Editing


http://www.youtube.com/watch?v=FVuAwBGw_pQ
mRNA Splicing

http://www.youtube.com/watch?v=YjWuVrzvZYA

mRNA Processing

https://www.youtube.com/watch?v=FVuAwBGw_pQ


RNA Splicing

(for other virtual cell animations visit http://vcell.ndsu.edu/animations/ )
 
8 Protein synthesis and Gene Regulation



 http://www.nature.com/scitable/topicpage/nucleic-acids-to-amino-acids-dna-specifies-935


http://www.youtube.com/watch?v=5bLEDd-PSTQ



Translation


http://www.youtube.com/watch?v=Ikq9AcBcohA

Protein Synthesis Animation Video



http://www.youtube.com/watch?v=TfYf_rPWUdY

mRNA Translation (Advanced)
optional extra: 
https://www.dnalc.org/

UNIT-II

1 Humoral and Cellular Immunology

 https://youtu.be/sYjtMP67vyk

 + power point presentations HUMORAL IMMUNOLOGY.pdf  + cellular responses.pdf

2 Structure and Functional Properties of Antibodies

 power point presentations ANTIBODIES.pdf

3 Production of Antibodies

 power point presentations ANTIBODIES.pdf


5 Complement System, Activation and Regulation

 https://youtu.be/d6qFPegEYV0

https://medlineplus.gov/lab-tests/c-reactive-protein-crp-test/

https://www.ncbi.nlm.nih.gov/books/NBK27100/

UNIT-III

1 Amino Acids, Proteins, Carbohydrates and Lipids: Structure and Functions

2 Metabolism of essential Amino Acids and Urea Cycle, Denovo and Salvage pathway for Nucleotide Synthesis

3 Bioenergetics, Glycolysis, Citric Acid Cycle, Oxidative Phosphorylation,

4 Oxidation of Fatty acids,

5 Gluconeogenesis

6 Hormonal Regulation

UNIT-IV

1 Introduction to Microbiolgy

2 Bacteria Structure and Function

3 Viruses and Prions

4 Eukaryotic pathogens

5 Fungi and Parasites

6 Microbial growth and Metabolism

7 Microbial genetics

8 Immunization, sterilization and disinfection

9 Fighting infections
 
 
 
Practicals
 
1. Detection, Isolation and Staining methods for different microorganisms of
forensic application

https://vlab.amrita.edu/?sub=3&brch=73&sim=1338&cnt=1

https://vlab.amrita.edu/?sub=3&brch=76&sim=1109&cnt=1

https://www.jove.com/v/10092/gram-staining-of-bacteria-from-environmental sources


2. Isolation of Plasmid DNA, Restriction enzyme digestion – ligation of plasmid
DNA
PLASMID ISOLATION: https://youtu.be/6fdZayzu2_U  
 https://vlab.amrita.edu/?sub=3&brch=77&sim=314&cnt=1   LIGATION: https://youtu.be/BYzaWI8zIVQ  
RESTRICTION ENZYME DIGESTION: https://youtu.be/L5Ikw5l6dZk 
 
3. Study (observation) of some pathogenic Fungi and bacteria, (permanent slides)
 

SLIDE CULTURE FOR FUNGI :

 

https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=1


4. Microbiological examination of water (Coli form test Microbiological
examination of milk
 

COLIFORM BACTERIAL ANALYSIS: https://www.youtube.com/watch?v=i0433lTS908 MILK EXAMINATION: https://vlab.amrita.edu/?sub=3&brch=73&sim=1630&cnt=1


5. Study of general Morphology and cultural characteristics of
staphylococeusStreptococcus, Darmatophytes etc.
 
https://vlab.amrita.edu/index.php?sub=3&brch=187&sim=324&cnt=2  STAPHYLOCOCCUS :  
https://www.intechopen.com/books/frontiers-in-i-staphylococcus-aureus-i- /staphylococcus-aureus-overview-of-bacteriology-clinical-diseases-epidemiology antibiotic-resistance  
STREPTOCOCCUS:  
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Streptococcus.htm  DERMATOPHYTES:  
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC172857/pdf/080240.pdf (PG 246)
 
 
6. Study of normal flora of human body
 

https://www.ncbi.nlm.nih.gov/books/NBK7617/

https://vlab.amrita.edu/?sub=3&brch=73&sim=213&cnt=1


7. Observation some spoiled food materials
 
https://youtu.be/yzo_sWnaTUo  
https://youtu.be/SNRMOSkOE_Q  
https://youtu.be/BlKP35bct2o 
 
8. Biochemical test for the identification of Soli bacteria (IMVIC test)
 
Voges-Proskauer Test : https://vlab.amrita.edu/?sub=3&brch=76&sim=215&cnt=2
 
 
9. Amino acid detections paper chromatography
 
https://royalsocietypublishing.org/doi/10.1098/rsos.160764

https://vlab.amrita.edu/?sub=3&brch=63&sim=154&cnt=1  
https://youtu.be/xXMYv_U8SDc 
 
 
10. Comparative evaluation of different methods of protein analysis, Lowry, Biuret,
Kjeldahl,UV
 
Lowry Assay : https://youtu.be/2Re34LhdbGw  
Kjedahl's Method: https://www.jove.com/t/5688/photometric-protein determination#:~:text=Photometric%20protein%20determination%20is%20based,all %20photometric%20protein%20determination%20methods  
Biuret Method : http://amrita.olabs.edu.in/?sub=73&brch=8&sim=140&cnt=2 
 
 
11. Specific reactions for carbohydrates and estimation
 
https://vlab.amrita.edu/?sub=3&brch=63&sim=631&cnt=2
https://youtu.be/Ewe7i1D9lSQ
 
 
12. Isolation of amino acids and proteins
 
https://vlab.amrita.edu/?sub=3&brch=63&sim=1094&cnt=1 ,  
https://vlab.amrita.edu/index.php?sub=3&brch=278&sim=1468&cnt=1  https://vlab.amrita.edu/index.php?sub=3&brch=278&sim=1464&cnt=1
 
 
13. Estimation of DNA and RNA

DNA Extraction:

https://vlab.amrita.edu/index.php?sub=3&brch=77&sim=1365&cnt=2 DNA Estimation: https://youtu.be/buDvwq8zECw

Simultaneous DNA-RNA Extraction: https://www.jove.com/t/54067/simultaneous dna-rna-extraction-from-coastal-sediments-quantification

 
 
 
 
 
 
https://www.vlab.co.in/broad-area-biotechnology-and-biomedical-engineering


https://mvii-au.vlabs.ac.in/exp/two-bacterial-unknowns/simulation.html

https://mvii-au.vlabs.ac.in/exp/litmus-milk-test/

https://mvii-au.vlabs.ac.in/exp/urease-test/

https://mvii-au.vlabs.ac.in/exp/triple-sugar-iron-agar/

http://mbvi-au.vlabs.ac.in/molecular-biology-1/Plasmid_Isolation/

http://mbvi-au.vlabs.ac.in/molecular-biology-1/Restriction_Digestion/

http://mbvi-au.vlabs.ac.in/molecular-biology-1/Agarose_Gel_Electrophoresis/

http://mbvii-au.vlabs.ac.in/molecular-biology-2/Ligation_Using_T4_DNA_Ligase/

http://mbvii-au.vlabs.ac.in/molecular-biology-2/Polymerase_Chain_Reaction/

http://cbi-au.vlabs.ac.in/cell-biology-1/Light_Microscope/

http://cbii-au.vlabs.ac.in/cell-biology-2/Mitosis_in_Onion_Root_Tips/

https://biotech01.vlabs.ac.in/exp/estimation-of-amino-acids/

https://biotech01.vlabs.ac.in/exp/adulteration-in-milk/

https://biotech01.vlabs.ac.in/exp/iodine-value-fats-oils/

https://biotech01.vlabs.ac.in/exp/analysis-of-amino-acid/

https://biotech01.vlabs.ac.in/exp/analysis-of-carbohydrates/
 
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