MFS-T3-C3: Forensic Biology

  UNIT-I
1. Forensic Biology:
General Concept and Definitions, History and Nature, Classification of different
biological evidences, Principals of collection and preservation of biological exhibits

Forensic Biology by Richard Li: pp. 39-47 (In 2nd Edition 3-30)
Advanced/Reference Material:
forensic pathology Forensic Science Central:
http://aboutforensics.co.uk/forensic-pathology/
2. Animals, Plants and Microorganisms in Legal Investigation

Forensic Botany & Geology:
https://web.archive.org/web/20150408033413/http://forensicsciencecentral.co.uk/botanygeology.shtml
Forensic Entomology
https://web.archive.org/web/20150325062044/http://forensicsciencecentral.co.uk/entomology.shtml
Forensic Pathology
https://web.archive.org/web/20150331072240/http://forensicsciencecentral.co.uk/pathology.shtml

 Microbes as forensic indicators (abstract only)
 http://www.ncbi.nlm.nih.gov/pubmed/23018494

 
Advanced/Reference Material:
Microbes as forensic indicators (full paper)
http://www.msptm.org/files/311_-_330_Alan_Gunn.pdf
 

3. Forensic Entomology: General entomology and arthropod biology

Forensic Entomology
http://aboutforensics.co.uk/forensic-entomology/
other: The use of insects in forensic investigations: An overview on the scope of forensic entomology
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296382/

Estimating the Post Mortem Interval, part 1 - Stage One of Decay (Fresh Decay)
https://www.youtube.com/watch?v=n92P0yr1mMU
Estimating Post Mortem Interval, part 2 - Stage Two of Decay (Bloat) 
https://www.youtube.com/watch?v=M4SuDHpqeUE
Estimating Post Mortem Interval, part 3 - Stage Three of Decay (Active Decay)
https://www.youtube.com/watch?v=r5ynpWVem0Y
Estimating Post Mortem Interval, part 4 - Stage Four of Decay (Dry Decay)
https://www.youtube.com/watch?v=uM4hMxub_sE
Estimating Post Mortem Interval, part 5 - Forensic Entomology 
 https://www.youtube.com/watch?v=Ie-_bTsxGJU
Estimating Post Mortem Interval, part 6 - Environmental Factors
 https://www.youtube.com/watch?v=YtRqRDJn2Ds
extra:
Forensic Entomology, with Chris Shreve
https://www.youtube.com/watch?v=pAlLkYSEfC4
 
4. Insects of forensic importance

Forensic Entomology India
http://www.forensicentomologyindia.com/
Insect Fauna of forensic importance from India
http://www.forensicentomologyindia.com/insect_fauna_of_forensic_importance
  5. Collection of entomological evidence during death investigations,
SFU Forensic Entomology
 https://www.youtube.com/watch?v=VeOtScDxq6s

SFU Forensic Entomology Part 2 
https://www.youtube.com/watch?v=Ov1tUK8r6sM
 

6. Insect succession on carrion and its relationship to determine time since death, its application to Forensic Entomology.

on maggots and murders forensic entomology:
http://www.nhm.ac.uk/resources-rx/files/22feat_maggots_and_murders-7201.pdf

 

UNIT-II
1. Forensic Paleontology: Pollen: Structure, function, methods of identification and comparison
Significance of Palynology in Forensic Problems:
http://www.indmedica.com/journals.php?journalid=9&issueid=67&articleid=856&action=article
Reference/Advanced material:
 Forensic palynology: Why do it and how it works
http://www.sciencedirect.com/science/article/pii/S0379073806005007
 FORENSIC PALYNOLOGY: A NEW WAY TO CATCH CROOKS
http://anthropologyworldnews.tamu.edu/faculty/bryant/publications/Bryant-Mildenhall-1998-New-Way-to-Catch-Crooks.pdf
 
2. Microbial Forensics: Applications in Bioterrorism, Building a National capacity
to investigate Bioterrorism, Database and Infectious microorganisms, Biocrimes
and Physican.
 
https://drive.google.com/file/d/1fnAXV7dUXU_xqCngjL_BDQ2liHuNjjh_/view?usp=sharing
 
https://pubmed.ncbi.nlm.nih.gov/23018494/
https://www.msptm.org/files/311_-_330_Alan_Gunn.pdf
 
 
3. Diatoms: Nature, location structure, extraction from various body tissues, including bone marrow, preparation of slides, methods of identification and comparison, forensic significance
Role of Diatoms in the World of Forensic Science
http://www.omicsonline.org/role-of-diatoms-in-the-world-of-forensic-science-2157-7145.1000181.pdf
 
other:
BSA's Classroom Plant Talking Point
http://botany.org/PlantTalkingPoints/Crime.php
 
 

 

 UNIT-III
Hair: Morphology of hair Cuticle cortex and medulla area of hair
2. Three phases of hair growth
3. Distinction between animal and human hair
4. Hair features useful for microscopic comparison of human hair

https://web.archive.org/web/20121110115842/https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2000/deedrick.htm/deedric1.htm

extra:Hair Analysis Notes
http://pjteaches.com/PDF/Forensics/Unit_6/HairAnalysisNotes.pdf 

Extra/Advanced/Reference Material:
 Hair and Fiber video lecture (13-09)
https://www.youtube.com/watch?v=Q2iO6CGxwcU
 Hair Evidence:
https://www.youtube.com/watch?v=LXQ3fX3N8Uo
 Hair- Forensic Science
https://www.youtube.com/watch?v=VxawTJpGOgk
 Microscopic Comparison of Hair and Fiber Evidence
https://www.youtube.com/watch?v=e3ZlA5GoFVw 
Britain's CSI School - Hair and Fibre - The One Show
https://www.youtube.com/watch?v=BjLHW7qQEl0 
forensic hair analysis review
https://www.youtube.com/watch?v=6vsAtujyxCc  

 
5. Wild Life Forensics: Introduction & importance

Critically endangered species brochure (page no. 1 only):
http://www.indiaenvironmentportal.org.in/files/critically%20endangered%20species.pdf
 

scientific institutes working in india on this field:
http://e-portal.ccmb.res.in/lacones/lacones.html
http://wccb.gov.in/

http://www.tracenetwork.org/
Protected and endangered species of animals and plants,
 endangered-species-of-india
http://www.slideshare.net/dhaveji/endangered-species-of-india
India: Our endangered wildlife - a cause for concernhttp://www.unodc.org/southasia//frontpage/2012/june/our-endangered-wildlife-a-cause-for-concern.html

India: Our endangered wildlife - a cause for concern: 
http://www.unodc.org/southasia//frontpage/2012/june/our-endangered-wildlife-a-cause-for-concern.html 

 
other (not compulsory):
Wildlife forensic science
https://en.wikipedia.org/wiki/Wildlife_forensic_science 

 
6 Introduction to conservation genetics

Conservation Genetics 2


7 Species or subspecies? Resolving taxonomic uncertainty

DNA barcoding


SUBSPECIES, SEMI SPECIES, SUPER SPECIES


8 Wild populations, captive populations and conservation management units

Bottlenecks and Founder Efects 


9 Genetics and Reproduction, Cloning, Genetic selection

Conservation Genetics 

 
10 Using molecular biology for species preservation

LaCONES


UNIT-IV (some part from book: pp39-43,85-147, 160-165)
1. Body Fluids: The nature of blood

Forensic Biology by Richard Li: pp. 85-87 (In 2nd Edition 231-233)

 
2. Chemical and Microscopic Analysis of Biological Stains

Forensic Biology by Richard Li: pp. 145-147 (In 2nd Edition 310-311)

 
3. Screening Evidence for Biological Stains in Forensic Casework


Forensic Biology by Richard Li: pp. 39-43
(In 2nd Edition 21-22)

 
4. Species of Origin and Serology Separation Techniques

Forensic Biology by Richard Li: pp. 101-112 (In 2nd Edition 145-155)

 
5. ABO Grouping and Secretor Status, List of ABO antigens and antibodies in blood

Forensic Biology by Richard Li: pp. 160-165 (In 2nd Edition 236-239)

 
6. Principles of heredity

any book or
https://www2.palomar.edu/anthro/mendel/mendel_1.htm
https://www.sciencelearn.org.nz/resources/2000-mendel-s-principles-of-inheritance

https://www.nature.com/scitable/topicpage/gregor-mendel-and-the-principles-of-inheritance-593/

https://en.wikipedia.org/wiki/Mendelian_inheritance

 


7. Biological Markers of Forensic Significance

Forensic Biology by Richard Li: pp.42-43 (In 2nd Edition 231-233)


8. Forensic characterization of blood, Preservation of suspected blood
Forensic Biology by Richard Li: pp.  88-95 (In 2nd Edition 232-241)

Human Blood Testing-  Presumptive Tests
https://www.youtube.com/watch?v=pnH2HnB-GrI
LUMINOL   INVESTIGACIÓN CSI
https://www.youtube.com/watch?v=zVFpxOvDTaw
https://www.youtube.com/watch?v=JIrVgF4CZTc
Kastle-Meyer Test
https://www.youtube.com/watch?v=sF0Z0jJGwX8
Hemastix Test
https://www.youtube.com/watch?v=Uax90QN91Zc

 
9. The nature of semen Forensic characterization of semen, Semen stains for
laboratory examination

Forensic Biology by Richard Li: pp. 115-130 (In 2nd Edition 257-271)
P30 Test
https://www.youtube.com/watch?v=E3COc2Thh4k
Acid Phosphatase Test
https://www.youtube.com/watch?v=tSgPqF32Zao

10. The nature of saliva, Forensic characterization of saliva

  Forensic Biology by Richard Li: pp.135-147 (In 2nd Edition 277-285)

 

 MFS-P1-C2: Forensic Biology (Pr) PRACTICAL

1. Sample preparation of hairs, human and animal hair morphology,
measurements (diameter, medullary ratio)


https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2000/deedrick.htm/deedric1.htm

extra:Hair Analysis Notes
http://pjteaches.com/PDF/Forensics/Unit_6/HairAnalysisNotes.pdf 

Extra/Advanced/Reference Material:
 Hair and Fiber video lecture (13-09)
https://www.youtube.com/watch?v=Q2iO6CGxwcU
 Hair Evidence:
https://www.youtube.com/watch?v=LXQ3fX3N8Uo
 Hair- Forensic Science
https://www.youtube.com/watch?v=VxawTJpGOgk
 Microscopic Comparison of Hair and Fiber Evidence
https://www.youtube.com/watch?v=e3ZlA5GoFVw 
Britain's CSI School - Hair and Fibre - The One Show
https://www.youtube.com/watch?v=BjLHW7qQEl0 
forensic hair analysis review
https://www.youtube.com/watch?v=6vsAtujyxCc 

 2. Human hair comparison, colour, treatment, pigment shape and distribution,
damage, disease, medulla, root, tip – Animal hair diameter, medulla, color
banding, scale casts
3. 4. Vegetable fiber characterization
Tests for saliva
5. Presumptive/confirmatory tests for blood and semen
6. RT PCR based identification of human tissue
7. DNA-barcoding based identification of plant species from trace sample

https://dnabarcoding101.org/files/using-dna-barcodes.pdf


8. DNA isolation from hair and bone

https://docs.google.com/document/d/1yAS1B4i_Jk4P4rzVtQPkeu1cyXtCiI_b/edit?usp=sharing&ouid=109175724865976975619&rtpof=true&sd=true

 Phadebas® Forensic Saliva Test Products
http://www.phadebas.com/products/forensic-saliva-test-products
 BVDA: Presumptive identification of semen - Sam... http://www.bvda.com/EN/sect6/en_6_2a.html
SALIVARY α-AMYLASE KINETIC ENZYME ASSAY KIT
https://www.salimetrics.com/assets/documents/1-1902.pdf
Red Starch | Sigma-Aldrich
 http://www.sigmaaldrich.com/catalog/product/sigma/94054?lang=en&region=IN
Sensitivity and specificity of presumptive tests for blood, saliva and
semen
http://link.springer.com/article/10.1007%2Fs12024-013-9515-6

Under the Microscope: Get Forensic with Hair Analysis
http://www.carolina.com/teacher-resources/Interactive/forensic-hair-analysis-activity/tr10879.tr
BVDA: Finding and identifying blood:
http://www.bvda.com/EN/sect6/en_6_1a.html

Isolation of DNA from Hair Follicles and Hair Shafts using
Norgen’s Dried Blood Spot Genomic DNA Isolation Kit 
https://norgenbiotek.com/sites/default/files/resources/dried_blood_spot_dbs_dna_isolation_kit_isolation_of_dna_from_hair_follicles_and_hair_shafts_using_norgens_dried_blood_spot_genomic_dna_isolation_kit_application_notes_401.pdf

FORENSIC BIOLOGY SECTION  CASEWORK TEST METHODS:
http://www.in.gov/isp/labs/files/Biology_Casework_Test_Method.pdf
biochemistry_workbook:
http://www.umb.edu.pl/photo/pliki/WL_jednostki/zaklad_biochemii_lekarskiej/pdf/biochemistry_workbook.pdf
Illustrated_Guide_to_Home_Forensic_Science_Experiments
http://www.thehomescientist.com/forensics/Illustrated_Guide_to_Home_Forensic_Science_Experiments.pdf
 ab102523 Amylase Assay Kit
http://www.abcam.com/ps/products/102/ab102523/documents/ab102523%20Amylase%20Assay%20kit%20protocol%20v5%20%28website%29.pdf
Forensic Biology Manual Directorate of Forensic Science, MHA, Govt. of India
(if you find a weblink to it plz paste in comments)
Bloody Prints:
https://www.youtube.com/watch?v=yE0ReXXe_sQ
Body-Fluid-SOPs-Rev-O6:
http://www.ncdoj.gov/getdoc/0c6b24c3-af68-4efa-a0e7-17a55f1b1147/Body-Fluid-SOPs-Rev-O6.aspx

Collecting Cigarette Evidence:
http://www.youtube.com/watch?v=sI-Q49sZiaQ
Swabbing Cigarettes
http://www.youtube.com/watch?v=nhf7_Zimp2M


Swabbing Cigarettess 2
https://www.youtube.com/watch?v=a575rm00Ph0



Swabbing Water Bottle
https://www.youtube.com/watch?v=x3sOk2DW6RU

Unit 4.3 Druggist Fold
https://www.youtube.com/watch?v=j1NMJUuRZMU

Cutting Blood from Shirt
Scraping Blood From Shirt
https://www.youtube.com/watch?v=fmeguTEWe6c

Microscopy of Hair Part II: A Practical Guide and Manual for Animal Hairs
https://www.fbi.gov/about-us/lab/forensic-science-communications/fsc/july2004/research/2004_03_research02.htm
  DNA Analyst Training : Bio Screening : blood
https://www.youtube.com/watch?v=8Q_LqRULkAw
DNA Analyst Training : Bio Screening : Saliva
https://www.youtube.com/watch?v=IkMSGmgn1hw

Presumptive Test for Blood (Kastle-Meyer Test)
http://flash.lakeheadu.ca/~lubiotec/Presumptive%20Test%20for%20Blood.pdf
Watkins_Brown_luminol_BS
http://www.bluestar-forensic.com/pdf/en/Watkins_Brown_luminol_BS.pdf


Reference Material:
Illustrated Guide to Home Forensic Science Experiments
 http://www.thehomescientist.com/forensics/Illustrated_Guide_to_Home_Forensic_Science_Experiments.pdf